In Vitro Culture System for Atherogenesis

An in vitro culture system for crayfish organs.

Organs of crayfishes--mainly integument of Astacus leptodactylus--have been kept in an in vitro culture of several days. The medium consists of 2 parts of van Harreveld saline and one part of horse serum, supplemented with antibiotics for long term cultures. This medium is very similar to the Astacus hemolymph in its ionic content, the pH, the osmolarity, the protein concentration and the chole...

Primary culture of ovarian follicular cells of Sterlet, Acipenser ruthenus to develop an in vitro system

The aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in Sterlet, Acipenser ruthenus. Oocytes for the primary culture were obtained from the ovaries of a 6 years old Sterlet 729 g in weight and 47 cm in total length. The oocytes were in advanced vitellogenesis stage (PI >10). A part of the ovary (containing about 300 follicles) w...

An Efficient In Vitro Culture System To Amplify Spermatogonia Stem Cell Markers

Background: Proliferation of spermatogonial stem cells (SSCs) can be a treatment for infertile men. Here, we design an efficient method based on culturing in the presence of Sertoli cells to improve the expression level of some specific spermatogonia stem cell genes during two weeks post culture. Materials and Methods: Cells were derived from neonatal (2-6 days old) mice testes and were cultur...

Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts

نظام سیاسی و فرهنگ مالیات politic system and tax culture

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